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Biological analyses were performed on PBMCs, assessing mitochondrial enzymology (citrate synthase and ETC complexes) and mitochondrial respiration using Seahorse XFe96 with the mitochondrial oxidative stress test
3.1.4 Ferroptosis in cerebral ischemia-reperfusion injury after ischemic stroke During cerebral ischemia-reperfusion, excessive ROS are generated, closely linking CIRI with ferroptosis activation by ROS (Liu et al., 2024b
Furthermore, HSPA5 is a molecular chaperone associated with endoplasmic reticulum stress that can bind to GPX4 to inhibit protein kinase-induced degradation of GPX4, thereby suppressing ferroptosis in pancreatic cancer cells
Key lipids, including ceramides, sphingomyelins, phospholipids, diacylglycerols, and triglycerides, as well as branch-chain amino acids, have been found to be dysregulated in both ME/CFS and GWS (Germain et al., 2018, 2020